Journal: Pharmaceuticals

Article Title: Low Immune Cross-Reactivity between West Nile Virus and a Zika Virus Vaccine Based on Modified Vaccinia Virus Ankara

doi: 10.3390/ph15030354

Figure Lengend Snippet: Experimental design, body weight loss, and survival analysis. ( A ) Experimental design. Four-week old CD1 female mice ( n = 10/group) were vaccinated with two doses of MVA-ZIKV encoding ZIKV prM and E proteins, or with MVA-WT as a control, at days 0 and 13 and challenged with WNV 27 days after the first immunization. Animals were monitored for body weight and survival after WNV challenge, and surviving animals were euthanized 14 days after challenge. ( B ) Body weight loss upon WNV infection. Data are presented as mean ± SEM. ( C ) Kaplan–Meier survival analysis.

Article Snippet: Groups of four-week-old Hsd:ICR CD1 female mice ( n = 10/group, Envigo) were immunized intraperitoneally (i.p.) with two doses (2 × 10 7 PFU/animal) of MVA-ZIKV or MVA-WT (used as control of immunization) at days 0 and 13.

Techniques: Control, Infection

Journal: Pharmaceuticals

Article Title: Low Immune Cross-Reactivity between West Nile Virus and a Zika Virus Vaccine Based on Modified Vaccinia Virus Ankara

doi: 10.3390/ph15030354

Figure Lengend Snippet: Analysis of antigen-specific humoral responses elicited in CD1 mice vaccinated with MVA-WT or MVA-ZIKV and challenged with WNV. ( A ) Induction of anti-ZIKV IgG antibodies in mice immunized with MVA-ZIKV or MVA-WT and analyzed by ELISA. Values correspond to the fold change over negative serum samples obtained before immunization. ( B ) Induction of anti-ZIKV neutralizing antibodies (nAbs). Data are expressed as the reciprocal serum dilution that neutralized 90% of ZIKV (PRNT 90 ). ( C ) Induction of anti-WNV IgG antibodies analyzed by ELISA. ( D ) Induction of anti-WNV nAbs. Each symbol in the graphs denotes a single animal. Three asterisks (***) denote statistically significant differences ( p < 0.0001) and n.s. indicates not statistically significant differences. Two-way ANOVA Tukey’s multiple comparison was performed with Graphpad Prism 7.0. For experimental design, see panel (A) in .

Article Snippet: Groups of four-week-old Hsd:ICR CD1 female mice ( n = 10/group, Envigo) were immunized intraperitoneally (i.p.) with two doses (2 × 10 7 PFU/animal) of MVA-ZIKV or MVA-WT (used as control of immunization) at days 0 and 13.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: British Journal of Pharmacology

Article Title: Inhibition of fatty acid amide hydrolase in the CNS prevents and reverses morphine tolerance in male and female mice

doi: 10.1111/bph.15031

Figure Lengend Snippet: Levels of mRNA from genes related to fatty acid ethanolamides in morphine‐tolerant mice. (a) Time course of the development of morphine tolerance in CD1 mice. (b) Transcriptional changes in Napepld, Faah, Ppara, Cnr1, and Cnr2 were evaluated in the spinal cord of the mice shown in (a). Results shown are the means ± SEM (n = 5 per group). *P < .05, significantly different from vehicle‐treated animals

Article Snippet: We used male and female CD1 mice weighing 25–30 g (Harlan Laboratories, Indianapolis, USA) and FAAH‐null (FAAH −/− ) mice (25–30 g), which had been backcrossed to C57BL/6J mice for more than 10 generations (Cravatt et al., 2001 ).

Techniques: